Search results for " Methionine"

showing 10 items of 14 documents

Exposure to Toxic Heavy Metals Can Influence Homocysteine Metabolism?

2019

Background: Homocysteine is a sulfur amino acid whose metabolism is activated in two pathways: remethylation to methionine, which requires folate and vitamin B12, and transsulfuration to cystathionine, which needs pyridoxal-5’-phosphate. High homocysteine level increases the risk of developing heart disease, stroke, peripheral vascular diseases, and cognitive impairment. Some evidence showed that exposure to these metals increased plasma homocysteine levels. Methods: A systematic review was carried out to clarify the relationship between homocysteine blood levels and exposure to toxic heavy metals (Lead, Cadmium, Mercury, and Chromium). Results: The results of this systematic review i…

0301 basic medicinemedicine.medical_specialtyHyperhomocysteinemiamercury6HomocysteinePhysiologycadmiumvitamin b<sub>6</sub>Clinical BiochemistryCadmium; Chromium; Folate; Lead; Mercury; Methionine; MTHFR; Vitamin B; 12; Vitamin B; 6TranssulfurationReview010501 environmental sciencesfolate01 natural sciencesBiochemistryvitamin B603 medical and health scienceschemistry.chemical_compoundInternal medicinemedicineVitamin B12Vitamin BMolecular Biology0105 earth and related environmental sciencesmethionineleadMethioninebiologybusiness.industrylcsh:RM1-950Cell BiologyMetabolismvitamin B12medicine.diseaseCystathionine beta synthase12lcsh:Therapeutics. Pharmacology030104 developmental biologyEndocrinologychemistryMethylenetetrahydrofolate reductaseMTHFRbiology.proteinchromiumbusinessvitamin b<sub>12</sub>Antioxidants
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Mboat7 down-regulation by hyper-insulinemia induces fat accumulation in hepatocytes.

2020

Background: Naturally occurring variation in Membrane-bound O-acyltransferase domain-containing 7 (MBOAT7), encoding for an enzyme involved in phosphatidylinositol acyl-chain remodelling, has been associated with fatty liver and hepatic disorders. Here, we examined the relationship between hepatic Mboat7 down-regulation and fat accumulation. Methods: Hepatic MBOAT7 expression was surveyed in 119 obese individuals and in experimental models. MBOAT7 was acutely silenced by antisense oligonucleotides in C57Bl/6 mice, and by CRISPR/Cas9 in HepG2 hepatocytes. Findings: In obese individuals, hepatic MBOAT7 mRNA decreased from normal liver to steatohepatitis, independently of diabetes, inflammatio…

Research paperTGFβ Transforming Growth Factor BetaIntracellular SpaceCRISPR Clustered Regularly Interspaced Short Palindromic RepeatshHEPS Human HepatocytesMice0302 clinical medicineLPIAT1DAG Diacylglyceroli.p. Intraperitonealmedia_commonFatty AcidsGeneral Medicine3. Good health030220 oncology & carcinogenesisHOMA-IR homeostasis Model Assessment of Insulin ResistanceMPO morpholinolcsh:Medicine (General)medicine.medical_specialtyPE Phosphatidyl-EthanolamineNashGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesTNFα tumor Necrosis Factor AlphaLDL Low Density LipoproteinsHyperinsulinismNAFLDSD Standard Dietmedia_common.cataloged_instanceHumansCPT1 Carnitine Palmitoyltransferase IPhosphatidylinositolGene SilencingEuropean unionVLDL Very Low Density Lipoproteinlcsh:RhHSC Human Hepatic Stellate Cellsmedicine.diseaseLipid MetabolismOA Oleic AcidCI Confidence IntervalMboat7 Membrane bound O-acyltransferase domain containing 7MCD methionine choline deficient diet030104 developmental biologyEndocrinologychemistryCDP Cytidine-DiphosphateFOXO1 Forkhead Box protein O1NAFLD nonalcoholic fatty liver diseaseSteatohepatitisBMI Body Mass IndexCL CardiolipinAcyltransferases0301 basic medicineAlcoholic liver diseaseCXCL10 C-X-C Motif Chemokine 10lcsh:Medicinechemistry.chemical_compoundNon-alcoholic Fatty Liver DiseaseIFG Impaired Fasting GlucoseAPOB Apolipoprotein BNonalcoholic fatty liver diseasePIP Phosphatidyl-Inositol-PhosphateSteatohepatitisqRT-PCR quantitative Real Time Polymerase Chain ReactionMice Knockoutlcsh:R5-920ORO Oil Red O StainingPI PhosphatidylinositolFatty liverTM6SF2 Transmembrane 6 Superfamily Member 2PhospholipidTAG TriglyceridesNASH Nonalcoholic SteatohepatitisLipogenesisLPA Lyso-Phosphatidic AcidPhosphatidylinositolSignal TransductionPS Phosphatidyl-SerinePA Palmitic AcidALD alcoholic liver diseasePC Phosphatidylcholinei.v. IntravenousFATP1 Fatty Acid Transport Protein 1Models BiologicalInternal medicinemedicineAnimalsNonalcoholic fatty liver diseasePPARα Peroxisome Proliferator-Activated Receptor alphaObesityG3P Glyceraldehyde-3-PhosphateSREBP1c Sterol Regulatory Element-Binding Protein 1HDL High Density Lipoproteinsbusiness.industryPI3K Phosphatidylinositol 3 KinaseMembrane ProteinsNHEJ Non-Homologues End JoiningPNPLA3 Patatin-like Phospholipase Domain-containing-3MTTP Microsomal Triglyceride Transfer ProteinLPIAT1 Lysophosphatidylinositol Acyltransferase 1TMC4 Transmembrane Channel-Like 4Disease Models AnimalGene Expression RegulationHepatocytesFOXA2 Forkhead Box A2mTOR mammalian target of RapamycinSteatosisInsulin ResistancebusinessPG Phosphatidyl-GlycerolFABP1 Fatty Acid-Binding Protein 1 FAS Fatty Acid SynthaseT2DM Type 2 Diabetes MellitusEBioMedicine
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METABOLIC PROMOTERS AFFECT YIELD AND FRUIT QUALITY OF 'SANFILIPPARA' AND 'TRABIA' LOQUAT

2015

Preliminary field trials with foliar applications of phenylalanine, methionine, oxylipins and sugars have shown improvements of external color, sugar content and uniformity of maturation in grapes, pome fruits, tomatoes and melons. In this study, we hypothesized that similar improvements could be obtained both in fruit of ‘Trabia’ and ‘Sanfilippara’ loquat (Eriobotrya japonica Lindl.) with applications of a commercial formulate called Sunred (Biolchim, Bologna, Italy) and containing various concentrations of the above metabolic promoters. The study was conducted on 12 ‘Sanfilippara’ and 12 ‘Trabia’ adult loquat trees grown in an orchard near Palermo, Italy. Half of the trees…

biologyfood and beveragesTitratable acidEriobotryaHorticulturebiology.organism_classificationJaponicaSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeHorticulturePomeYield (wine)CultivarOrchardEriobotra japonica fruit ripening methionine oxylipins peel color phenylalanineSugarActa Horticulturae
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Reconstructing the free-energy landscape of Met-enkephalin using dihedral principal component analysis and well-tempered metadynamics

2013

Well-Tempered Metadynamics (WTmetaD) is an efficient method to enhance the reconstruction of the free-energy surface of proteins. WTmetaD guarantees a faster convergence in the long time limit in comparison with the standard metadynamics. It still suffers however from the same limitation, i.e. the non trivial choice of pertinent collective variables (CVs). To circumvent this problem, we couple WTmetaD with a set of CVs generated from a dihedral Principal Component Analysis (dPCA) on the Ramachadran dihedral angles describing the backbone structure of the protein. The dPCA provides a generic method to extract relevant CVs built from internal coordinates. We illustrate the robustness of this …

Protein ConformationSurface PropertiesEnkephalin MethionineFOS: Physical sciencesGeneral Physics and AstronomyDihedral angle01 natural scienceslaw.invention03 medical and health scienceslaw0103 physical sciencesComputer SimulationCartesian coordinate systemPhysics - Biological PhysicsStatistical physicsPhysical and Theoretical ChemistryProtein secondary structureReference modelComputingMilieux_MISCELLANEOUS030304 developmental biologyMathematicsPrincipal Component AnalysisQuantitative Biology::Biomolecules0303 health sciences010304 chemical physicsMetadynamicsEnergy landscapeBiomolecules (q-bio.BM)Condensed Matter - Other Condensed Matter[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistryQuantitative Biology - BiomoleculesBiological Physics (physics.bio-ph)FOS: Biological sciencesPrincipal component analysis[ CHIM.THEO ] Chemical Sciences/Theoretical and/or physical chemistryPhysics::Accelerator PhysicsThermodynamicsEnergy MetabolismAlgorithmsOther Condensed Matter (cond-mat.other)Ramachandran plot
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Chronic ethanol feeding causes oxidative stress in rat liver mitochondria. Prevention by S-adenosyl methionine

1999

Rat liver mitochondriaGeneral MedicineGlutathioneGPX4Ethanol feedingmedicine.disease_causeBiochemistryLipid peroxidationchemistry.chemical_compoundBiochemistrychemistrymedicineS-Adenosyl methionineOxidative stressFree Radical Research
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Effect of metabolic promoters on yeld and fruit quality of 'Sanfilippara' and 'Trabia' loquat.

2014

Settore AGR/03 - Arboricoltura Generale E Coltivazioni Arboreefruit maturation methionine oxylipins peel color phenylanine.
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Synthesis, Stability and Relaxivity of TEEPO-Met: An Organic Radical as a Potential Tumour Targeting Contrast Agent for Magnetic Resonance Imaging

2018

Cancer is a widespread and life-threatening disease and its early-stage diagnosis is vital. One of the most effective, non-invasive tools in medical diagnostics is magnetic resonance imaging (MRI) with the aid of contrast agents. Contrast agents that are currently in clinical use contain metals, causing some restrictions in their use. Also, these contrast agents are mainly non-specific without any tissue targeting capabilities. Subsequently, the interest has notably increased in the research of organic, metal-free contrast agents. This study presents a new, stable organic radical, TEEPO-Met, where a radical moiety 2,2,6,6-tetraethylpiperidinoxide (TEEPO) is attached to an amino acid, methio…

116 Chemical sciencesPharmaceutical ScienceContrast Media01 natural sciencesAnalytical Chemistrylaw.inventionGLUCOSENuclear magnetic resonancePiperidineslawHeterocyclic CompoundsNeoplasmsDrug DiscoveryMoietymagneettitutkimusSpin labelElectron paramagnetic resonanceta116orgaaniset yhdisteetmedicine.diagnostic_testMolecular StructureChemistryPhantoms ImagingRelaxation (NMR)radicalsMagnetic Resonance Imaging3. Good healthChemistry (miscellaneous)Molecular MedicineMRIRadical010402 general chemistryvapaat radikaalitImaging phantomArticleCyclic N-OxidesvarjoainetutkimusnitroxidesmedicineAnimalsHumanscontrast agentstyppiyhdisteetPhysical and Theoretical Chemistrymethionine010405 organic chemistryOrganic ChemistryElectron Spin Resonance SpectroscopyMagnetic resonance imagingIN-VITRO3126 Surgery anesthesiology intensive care radiologynitroxides; radicals; methionine; contrast agents; MRI; NMR; EPRIn vitroNMRTRANSPORT0104 chemical sciencesREDUCTIONSPIN-LABEL1182 Biochemistry cell and molecular biologyEPRMolecules; Volume 23; Issue 5; Pages: 1034
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Maintenance of glutathione levels and its importance in epigenetic regulation.

2014

Glutathione (GSH) is present in almost all cell types playing an important function in organisms. It is the main antioxidant in many cell types and it also regulates the function of proteins, including transcription factors (reviewed in Pallardo et al., 2009; Markovic et al., 2010; Garcia-Gimenez et al., 2013a). Over recent years, growing evidence has suggested a link between GSH metabolism and the control of epigenetic mechanisms. Epigenetics is defined as the mitotically/meiotically heritable changes in gene expression that are not due to changes in the primary DNA sequence. This link between GSH and epigenetics occurs at different levels. Hence, GSH can affect DNA and histone methylation…

PharmacologyMethyltransferaseDNA methylationbiologyS-adenosyl methionineOpinion Articleepigenetic regulationmental disordersHistone H3chemistry.chemical_compoundBiochemistrychemistryprodrugsHistone methyltransferaseHistone methylationbiology.proteinHistone codePharmacology (medical)EpigeneticsMethionine synthaseS-Adenosyl methionineglutathioneglutathionylationFrontiers in pharmacology
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Brain met-enkephalin immunostaining after subacute and subchronic exposure to benzene

1994

Benzene is used in a wide variety of domestic and occupational activities, and due to its lipophilic nature, it accumulates in lipid-rich tissues like the brain. In this sense, neurotoxic action has long been associated with organic solvent exposure and it has been shown that benzene, injected in a single dose or during a prolongued administration, modifies the content of dopamine, noradrenaline, serotonin and its main metabolite 5-hydroxy indolacetic acid, in several brain regions of the rat, then revealing a stimulating action on brain monoamine synthesis and turnover. However, information concerning neurotoxic action of benzene exposure in vivo on peptidergic neuromodulatory systems is s…

MaleMet-enkephalinmedicine.medical_specialtyTime FactorsEnkephalin MethionineHealth Toxicology and MutagenesisCentral nervous systemNeuropeptideBiologyToxicologyRats Sprague-Dawleychemistry.chemical_compoundDopamineInternal medicineImage Processing Computer-AssistedmedicineAnimalsBrain ChemistryStaining and LabelingProteolytic enzymesBrainBenzeneGeneral MedicinePollutionRatsEndocrinologyMonoamine neurotransmittermedicine.anatomical_structurechemistryImmunologic TechniquesSerotoninImmunostainingmedicine.drugBulletin of Environmental Contamination and Toxicology
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Methionine Cycle Rewiring by Targeting miR-873-5p Modulates Ammonia Metabolism to Protect the Liver from Acetaminophen

2022

Drug-induced liver injury (DILI) development is commonly associated with acetaminophen (APAP) overdose, where glutathione scavenging leads to mitochondrial dysfunction and hepatocyte death. DILI is a severe disorder without effective late-stage treatment, since N-acetyl cysteine must be administered 8 h after overdose to be efficient. Ammonia homeostasis is altered during liver diseases and, during DILI, it is accompanied by decreased glycine N-methyltransferase (GNMT) expression and S-adenosylmethionine (AdoMet) levels that suggest a reduced methionine cycle. Anti-miR-873-5p treatment prevents cell death in primary hepatocytes and the appearance of necrotic areas in liver from APAP-adminis…

drug-induced liver injury (DILI); acetaminophen (APAP); ammonia; methionine cycle; miR-873-5p; therapy; polyamines; mitochondriatherapyacetaminophen (APAP)Bioquímica clínicaPhysiologypolyaminesClinical Biochemistrydrug-induced liver injury (DILI)Cell BiologyammoniamiR-873-5pBiochemistrymitochondriaParacetamolmethionine cycleMolecular BiologyAntioxidants
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